Investigation of the Cytotoxic Activity of Elettaria Cardamomum Extract with Different Cancer Drugs Combination on Breast Cancer Cell Lines

Abstract

Cancer is a fatal disease that has been going on from the past to the present. Breast cancer is one of the most common types of cancer. Breast cancer occurs when healthy cells in the breast tissue proliferate uncontrollably as a result of exposure to various factors. There are many studies on treatment approaches against breast cancer. However, while existing treatment methods kill cancer cells, they also damage healthy cells. Due to these harmful effects of current treatment methods, herbal treatment approaches are gaining importance for cancer therapies day by day. It is stated in the literature that Diindolylmethane (DIM) and indole-3-carbinol (I3C) molecules found in Elettaria cardamomum (E. cardamomum) extract have a cytotoxic effect by affecting the hormone pathway. In this study, the effect of E. cardamomum and drug combinations on MDA-MB-231, MCF-7 and J774 was investigated. The study aimed to compare the data in the literature by using both hormone-positive (MCF-7) and triple-negative (MDA-MB-231) breast cancer cell lines. As a result, it was determined that extract had higher cytotoxicity on MCF-7 compared to the MDA-MB-231, due to the presence of I3C and DIM molecules in E. cardamomum extract. In drug combinations, the highest cytotoxicity data was obtained in the Tamoxifen-E.cardamomum extract combination.