The Therapeutic Effect of Platelet-Rich Fibrin, Curcumin and Ozone Among Oral Wound Healing Process

Abstract

Background: It is difficult to treat the wounds in the mouth for whatever reason, especially in the combination of mucosa and hard palate. In our study, we discuss which have been shown to have positive effects on wound healing in the mouth, whether platelet-rich fibrin, ozone therapy and curcumin treatment are superior to each other.

Aim: We aimed to demonstrate its effectiveness in oral wounds by evaluating epithelial and mucosal cell regeneration and healing histopathologically.

Methods: We used fifty-one Wistar Hannover rats in our study. The study was designed in 5 groups, including 9 rats in each group. 6 rats were used as donors to obtain PRF. In group 1, only palate defect was created as the control, while PRF was applied locally to the 2nd group, Ozone to the 3rd group, Emu to the 4th group, and the combination of Curcumin and Emu to the 5th group. On the 15th day after the application, all palatine specimens were sent for histopathological examination. The results obtained were made using the ANOVA test for intergroup comparison. For statistical evaluation, the Kruskal-Wallis test and then Mann-Whitney U test were used.

Results: When wound healing is examined in total, epithelial thickness, regeneration, granulation, and collagen formation; While Ozone, PRF and Curcumin were also good, they were the least in the control group. While the most prominent vascularization was observed in the PRF and Emu groups, it was observed the least in the Ozone and Curcumin groups. There was no histopathological difference between the groups in terms of sufficient tissue formation.

Conclusion: Repair of intraoral defects is difficult in every sense. In addition to surgery, a lot of topical things have been tried. In the literature, there are studies on intraoral wound healing, especially cleft palate, showing the topical therapeutic effect of Ozone, Curcumin and PRF. However, in addition to surgical interventions, additional studies are needed for such topical applications to have a positive effect on wound healing. There is still a gap in the literature about which of the Ozone, PRF and Curcumin is more suitable as a topical supplement and at which stage of wound healing it is more effective.