Immunoregulatory Effect of DF-MSCs Derived from Lymphocytes of Children on Patients with Asthma

Abstract

Aim: This study aims to develop regenerative medicine and support the formation of new resources in terms of health tourism. We investigated the immunomodulatory effects of human dental follicle mesenchymal stem cells (hDF-MSCs) pre-stimulated with/without IFN-γ; in Derp p1 + asthma patients lymphocytes in-vitro.

Materials and methods: Dental follicles were obtained from University of Marmara Faculty of Dentistry Oral and Maxillofacial Surgery Department. Dental follicle derived MSCs were isolated, characterized and differentiated into osteogenic, adipogenic, chondrogenic linageages. Peripheral blood mononuclear cells (PBMC) were isolated from healty (hPBMC) and asthma patients (aPBMC). They were cultured with/without hDF-MSCs. All cultures were stimulated with Derp1, CDmix pre and post stimulated IFN-γ. Lymphocyte proliferation, Annexin V/PI apoptosis and CD4+CD25+FoxP3+ Treg ratio were analyzed after 72 hours of culture period.

Results: hDF-MSCs suppressed CD4+T cell proliferation significantly (p<0.05). Previously stimulated hDF-MSCs with IFN-γ suppressed CD4+T lymphocyte proliferation more than unstimulated MSCs (p=0.01). hDF-MSCs increased Treg ratio significantly (p<0.05). IFN-γ -stimulation of hDF-MSCs enhanced Treg ratio compared to un-stimulated MSCs (p=0.01). Both IFN-γ stimulated and un-stimulated hDF-MSCs suppressed lymphocyte apoptosis significantly (p<0.05). In accordance with the results, it would be appropriate to use IFN-γ stimulated hDFMSC’s in the treatment of inflammatory and allergic diseases to modulate immune responses.