Investigation of VKORC1 Gene Polymorphism in Patients with Bleeding Complaints due to Warfarin

Abstract

Objective: In the current study, the effect of genetic mutation was investigated on the patients referred to the emergency department due to the complaints of bleeding which receiving oral anticoagulant.

Method: The blood samples of 80 patients referred to emergency department with the complaints of bleeding who was using oral anticoagulants with any reason and of 40 healthy individuals were stored. The DNA was obtained from each sample with PCR amplification method and VKORC1 gene polymorphisms were scanned in DNA samples.

Results: The patient and the control group were consisted of similar age and gender. On the scanning of VCOR gene, homozygote mutation was detected in 4 (5%) patients, while heterozygote mutation was defined in 51 (64%) of the cases and normal genotype were in 25
(31%) cases in patient group. Heterozygote mutation was found at 28 (70%) individual and normal genotype were detected at 12 (30%) individual in control group.

Conclusion: Warfarin is one of the important treatment modalities for the antithrombotic therapy in the thrombotic events. However, laboratory follow-up requirement for bleeding, requires a serious follow-up during the use. Clarification of the genetic basis of the relationship between bleeding and dose setting will be increased the quality of life in the patients that treated with antithrombotic agents.